Journal
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
Volume 46, Issue -, Pages 328-336Publisher
ELSEVIER
DOI: 10.1016/j.etap.2016.08.009
Keywords
Nuclear receptors; Endocrine disruption; Ethinylestradiol; Testosterone; Fish; Liver
Funding
- European Regional Development Fund (ERDF) through COMPETE-Operational Competitiveness Programme
- POPH Operational Human Potential Programme
- national funds through FCT Foundation for Science and Technology [PTDC/CVT/115618/2009, SFRH/BPD/97139/2013, PEst-C/MAR/LA0015/2013]
- [UID/Multi/04423/2013]
- Fundação para a Ciência e a Tecnologia [UID/Multi/04423/2013, PTDC/CVT/115618/2009] Funding Source: FCT
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Peroxisome proliferator-activated receptor gamma (PPAR gamma) is a pivotal regulator of lipid and glucose metabolism in vertebrates. Here, we isolated and characterized for the first time the PPAR gamma gene from brown trout (Salmo trutta f. fario). Hormones have been reported to interfere with the regulatory function of PPAR gamma in various organisms, albeit with little focus on fish. Thus, primary hepatocytes isolated from juveniles of brown trout were exposed to 1, 10 and 50 mu M of ethinylestradiol (EE2) or testosterone (T). A significant (3 fold) decrease was obtained in response to 50 mu M of EE2 and to 10 and 50 mu M of T (13 and 14 folds), while a 3 fold increase was observed at 1 mu M of EE2. Therefore, trout PPAR gamma seems a target for natural/synthetic compounds with estrogenic or androgenic properties and so, we advocate considering PPAR gamma as another alert sensor gene when assessing the effects of sex-steroid endocrine disruptors. (C) 2016 Elsevier B.V. All rights reserved.
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