Punicalagin alleviates hepatotoxicity in rats challenged with cyclophosphamide

This study investigated the possible hepatoprotection of punicalagin in rats received cyclophosphamide (20 mg/kg/day, i.p., for 7 days). Punicalagin given at two doses, 15 and 30 mg/kg/day, p.o., for 7 days, starting the same day of cyclophosphamide administration. Punicalagin significantly and dose-dependently reduced the elevations of serum alanine aminotransferase, and liver nuclear factor-kappa B p65, tumor necrosis factor-alpha, interleukin-1 beta, malondialdehyde, nitric oxide, Bax/Bcl-2 ratio, inducible nitric oxide synthase, caspases 3 and 9 activities, and prevented the decrease of hepatic total antioxidant capacity. Punicalagin also attenuated the histopathological liver tissue damage, and decreased cyclooxygenase-2 expression in liver of rats received cyclophosphamide in a dose-dependent manner. It was concluded that punicalagin protected rat liver against cyclophosphamide toxicity by inhibiting oxidative/nitrosative stress, inflammation, and apoptosis. (C) 2016 Elsevier B.V. All rights reserved.