Journal
APPLIED SOIL ECOLOGY
Volume 160, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.apsoil.2020.103839
Keywords
Bacillus thuringiensis; Phosphate-solubilizing bacteria; Entomopathogens
Categories
Funding
- [PE INTA PNPV 1135033]
- [2019-PD-E4-I069-001]
Ask authors/readers for more resources
Phosphate-solubilizing strain INTA 293-1 from Corrientes province in Argentina has been identified as Bacillus thuringiensis, showing insecticidal activity against lepidopteran and dipteran larvae. It is capable of solubilizing different sources of inorganic phosphates, with solubilization associated with pH reduction. The strain contains cry1Ac, cry1Ia, cry2Aa, cry2Ab and vip3Aa genes.
Phosphate-solubilizing strain INTA 293-1 from a soil sample of Corrientes province (Argentina) was identified as Bacillus thuringiensis based on its faculty to produce crystalline inclusions during sporulation and 16S rDNA sequence similarity. INTA 293-1 was found to produce bipyramidal and cuboidal crystals, containing major proteins of ca. 130 and 65 kDa respectively, resembling those present in Lepidoptera- and Diptera-active strains. Accordingly, INTA 293-1 showed insecticidal activity against lepidopteran and dipteran larvae. No insecticidal activity nor evident effects on the normal development of coleopteran larvae or nematodes were observed. Screening of cry, cyt and vip genes showed that INTA 293-1 contains cry1Ac, cry1Ia, cry2Aa, cry2Ab and vip3Aa genes. Furthermore, this new entomopathogenic strain of B. thuringiensis was able to solubilize different sources of inorganic phosphates in liquid media following similar solubilization kinetics. INTA 293-1 was effective in solubilizing hydroxyapatite, patagonic phosphate rock, and to a lesser extent the aluminium phosphate. The differential behaviour between them may be explained by differences in INTA 293-1 survival. Phosphate solubilization was associated with pH reduction. This study reveals a little-explored aspect of B. thuringiensis and paves the way for the development of new agrobiological products with multiple modes of action.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available