Discovery and genetic identification of amphiphilic coprogen siderophores from Trichoderm hypoxylon

Siderophores are small molecular iron chelators and participate in the multiple cellular processes in fungi. In this study, we discovered and identified five amphiphilic coprogen siderophores including three new natural products according to LC-MS-guided separation strategy from Trichoderm hypoxylon. The structures of three new coprogens were elucidated by NMR spectroscopy, and high-resolution (HR)-ESI-MS analysis. Genetic deletions of dfcA and dfcB abolished the production of compounds 1-5 that implied their involvement in the biosynthesis of coprogens. Interestingly, cultivations of Delta dfcA and Delta dfcB mutants with pathogenic fungi Fusarium oxysporum and Mucor corcinelloides showed the weaker inhibitions in comparison to wild type that demonstrated coprogen's role in combating the pathogenic fungi. Our study not only enriched the diversities of siderophores but also provided an approach for finding the rare amphiphilic coprogen siderophores in fungi. Furthermore, this work provided a basis for investigation on the biosynthesis of fungal amphiphilic siderophores and their ecological roles in nature.

Automated summary

Siderophores are small molecular iron chelators involved in cellular processes in fungi. This study discovered and identified five amphiphilic coprogen siderophores, including three new natural products, from Trichoderma hypoxylon. Genetic deletions of dfcA and dfcB genes confirmed their involvement in coprogen biosynthesis and their role in combating pathogenic fungi.