Journal
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
Volume 36, Issue 4, Pages 947-951Publisher
WILEY
DOI: 10.1002/etc.3608
Keywords
Enzymatic digestion; Fourier transform infrared (FT-IR); Microplastics; Mussels; Emerging pollutants; Environmental toxicology
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Funding
- Marie Curie fellowship [PIEF-GA-2013-625915]
- Marine Alliance for Science and Technology Scotland Small Grant (Themes Forums) [BFSSG7]
- Natural Environment Research Council [NE/N006526/1] Funding Source: researchfish
- NERC [NE/N006526/1] Funding Source: UKRI
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The authors compared procedures for digestion of mussel soft tissues and extraction of microplastics. Complete tissue digestion was achieved with 1M NaOH, 35% HNO3, and protease at 9.6 UHb/mL (unit hemoglobin per mL); but use of HNO3 caused unacceptable destruction of some microplastics. Recovery of microplastics spiked into mussels was similar (93 +/- 10%) for NaOH and enzyme digestions. The authors recommend use of industrial enzymes based on digestion efficiency, microplastic recovery, and avoidance of caustic chemicals. (C) 2016 SETAC
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