Journal
ENVIRONMENTAL TOXICOLOGY
Volume 32, Issue 1, Pages 344-352Publisher
WILEY
DOI: 10.1002/tox.22239
Keywords
bisphenol A; superoxide dismutase; MnTBAP; human bone mesenchymal stem cells; beta-catenin; GSK3 beta
Categories
Funding
- Korea Research Foundation grant - Korean Government (MEST) [2010-0029355]
- Korea Research Institute of Bioscience and Biotechnology
- Korea Research Council of Fundamental Science and Technology (KRIBB/KRCF Research Initiative Program
- NAP)
- National Research Foundation of Korea [2010-0029355] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ask authors/readers for more resources
Bisphenol A (BPA), used in the manufacture of products based on polycarbonate plastics and epoxy resins, is well known as an endocrine-disrupting monomer. In the current study, BPA increased cytotoxicity in hBMSCs in a dose-and time-dependent manner, concomitantly with increased lipid peroxidation. Increased cell death in BPA-treated cells was markedly blocked by pretreatment with the superoxide dismutase mimetic MnTBAP and MnTMPyP, but not by catalase, glutathione, the glutathione peroxidase mimetic ebselen, the NOS inhibitor NAME, or the xanthine oxidase inhibitor allopurinol. Furthermore, the decline in nuclear beta-catenin and cyclin D1 levels in hBMSCs exposed to BPA was reversed by MnTBAP treatment. Finally, treatment of hBMSCs with the GSK3 beta inhibitor LiCl2 increased nuclear beta-catenin levels and significantly attenuated cytotoxicity compared with BPA treatment. Our current results in hBMSCs exposed to BPA suggest that BPA causes a disturbance in beta-catenin signaling via a superoxide anion overload. (C) 2016 The Authors Environmental Toxicology Published by Wiley Periodicals, Inc.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available