Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 60, Issue 28, Pages 15418-15425Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202101190
Keywords
activatable probes; analytical methods; fluorogenic imaging; orthotopic hepatocellular carcinoma; specific substrates
Categories
Funding
- National Research Foundation of Korea (NRF) - Korea government (MSIP) [2012R1A3A2048814]
- National Natural Science Foundation of China [21864011, 21775162]
- Hainan Key Research and Development Project [ZDYF2020133]
Ask authors/readers for more resources
This study successfully developed a near-infrared two-photon fluorescent probe with high selectivity for imaging the HCC-related biomarker CE in vivo and in situ. The probe correlated well with tumor apoptosis, enabling accurate evaluation of HCC treatment status. This advancement may contribute to precision imaging-guided diagnosis and treatment evaluation of HCC in situ.
Selective fluorescence imaging of biomarkers in vivo and in situ for evaluating orthotopic hepatocellular carcinoma (HCC) chemotherapy remains a great challenge due to current imaging agents suffering from the potential interferences of other hydrolases. Herein, we observed that carbamate unit showed a high selectivity toward the HCC-related biomarker carboxylesterase (CE) for evaluation of treatment. A near-infrared two-photon fluorescent probe was developed to not only specially image CE activity in vivo and in situ but also target orthotopic liver tumor after systemic administration. The in vivo signals of the probe correlate well with tumor apoptosis, making it possible to evaluate the status of treatment. The probe enables the imaging of CE activity in situ with a high-resolution three-dimensional view for the first time. This study may promote advances in optical imaging approaches for precise imaging-guided diagnosis of HCC in situ and its evaluation of treatment.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available