Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 60, Issue 26, Pages 14429-14437Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202102833
Keywords
anti-biofouling; brain; microfiber arrays; reversibility; stroke
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Funding
- NSFC [21635003, 21811540027]
- Innovation Program of Shanghai Municipal Education Commission [201701070005E00020]
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Understanding physiological and pathological processes in the brain requires tracking reversible changes in chemical signals with long-term stability. A new anti-biofouling microfiber array was developed to quantify extracellular Ca2+ concentrations and neuron activity in real-time across multiple regions in the mammalian brain for 60 days, providing high tempo-spatial resolution and the ability to monitor reversible changes during ischemia-reperfusion processes. This microarray serves as a versatile tool for investigating brain dynamics during pathological processes and drug treatment, demonstrating the significant influence of ROS on Ca2+ overload and neuron death after stroke.
Understanding physiological and pathological processes in the brain requires tracking the reversible changes in chemical signals with long-term stability. We developed a new anti-biofouling microfiber array to real-time quantify extracellular Ca2+ concentrations together with neuron activity across many regions in the mammalian brain for 60 days, in which the signal degradation was < ca. 8 %. The microarray with high tempo-spatial resolution (ca. 10 mu m, ca. 1.3 s) was implanted into 7 brain regions of free-moving mice to monitor reversible changes of extracellular Ca2+ upon ischemia-reperfusion processes. The changing sequence and rate of Ca2+ in 7 brain regions were different during the stroke. ROS scavenger could protect Ca2+ influx and neuronal activity after stroke, suggesting the significant influence of ROS on Ca2+ overload and neuron death. We demonstrated this microarray is a versatile tool for investigating brain dynamic during pathological processes and drug treatment.
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