Journal
ANALYTICAL LETTERS
Volume 55, Issue 1, Pages 1-10Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/00032719.2021.1907588
Keywords
Extraction; metabolomics; milk; proton nuclear magnetic resonance (1H-NMR)
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Funding
- MBIE NZ-China Grant: Integrated Tracing Technologies for Dairy Products [UOOX1501]
- University of Otago PhD Scholarship
- New Zealand Ministry of Business, Innovation & Employment (MBIE) [UOOX1501] Funding Source: New Zealand Ministry of Business, Innovation & Employment (MBIE)
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This study focused on milk metabolomics, with comparisons of extraction procedures and identification of the most efficient system. Through optimization of milk quantity and extraction procedure, the best precision and high-quality spectra were obtained. The study provides a sample preparation protocol for milk metabolomics that may be applicable to other samples.
Milk is a favorable for humans due to its nutritional composition. Metabolites in milk relevant to human health have received increased attention. Proton nuclear magnetic resonance (H-1-NMR) is commonly used for water-soluble metabolites due to rapid data acquisition and excellent precision. Extraction to separate fats and proteins from the metabolites is necessary to obtain high quality spectra. In the present work, various extraction procedures were compared for H-1-NMR. The mass of milk for extraction was also optimized. Overall, 2/1 (v/v) methanol/skim milk was selected to be the most efficient system. The best precision was obtained for 500 to 600 mg milk powder redissolved in 2 mL water and 0.5 mL skim milk for the subsequent extraction. This study provides a sample preparation protocol for milk metabolomics that may be appropriate for other samples.
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