Journal
ANALYTICAL CHEMISTRY
Volume 93, Issue 17, Pages 6739-6745Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c00438
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- PerkinElmer (Torion Division, American Fork, Utah)
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This paper compares static thermal gradient gas chromatography to isothermal GC using a stochastic transport model to simulate peak characteristics for C12-C14 hydrocarbons. The simulations show that a linear thermal gradient improves resolution significantly, while a static, non-linear thermal gradient provides the largest observed gains in resolution. Optimized linear thermal gradient conditions are shown to improve resolution by as much as 8.8% over comparative isothermal conditions.
This paper compares static (i.e., temporally unchanging) thermal gradient gas chromatography (GC) to isothermal GC using a stochastic transport model to simulate peak characteristics for the separation of C12-C14 hydrocarbons resulting from variations in injection bandwidth. All comparisons are made using chromatographic conditions that give approximately equal analyte retention times so that the resolution and number of theoretical plates can be clearly compared between simulations. Simulations show that resolution can be significantly improved using a linear thermal gradient along the entire column length. This is mainly achieved by partially compensating for loss in resolution from the increase in mobile phase velocity, which approximates an ideal, basic separation. The slope of the linear thermal gradient required to maximize resolution is a function of the retention parameters, which are specific to each analyte pair; a single static, thermal gradient will not affect all analytes equally. A static, non-linear thermal gradient that creates constant analyte velocities at all column locations provides the largest observed gains in resolution. From the simulations performed in this study, optimized linear thermal gradient conditions are shown to improve the resolution by as much as 8.8% over comparative isothermal conditions, even with a perfect injection (i.e., zero initial bandwidth).
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