Journal
ANALYTICAL CHEMISTRY
Volume 93, Issue 12, Pages 5277-5283Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c00322
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Funding
- Grant Agency of the Czech Republic [GACR EXPRO: 19-26896X]
- MEYS CR [LM2018110]
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This study introduces a 3D-printed electrochemical chiral sensor functionalized with magnetic COF and BSA, showing excellent chiral recognition ability towards L-Trp compared to D-Trp. The sensor can be used for determining the concentration of L-Trp in a racemic mixture. This on-demand fabrication of protein-COF-modified electrodes offers new approaches for enantiomer recognition.
Three-dimensional (3D) printing technology has attracted great attention for prototyping different electrochemical sensor devices. However, chiral recognition remains a crucial challenge for electrochemical sensors with similar physicochemical properties such as enantiomers. In this work, a magnetic covalent organic framework (COF) and bovine serum albumin (BSA) (as the chiral surface) functionalized 3D-printed electrochemical chiral sensor is reported for the first time. The characterization of the chiral biomolecule-COF 3D-printed constructure was performed by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and energy-dispersive Xray spectroscopy (EDX). A tryptophan (Trp) enantiomer was chosen as the model chiral molecule to estimate the chiral recognition ability of the magnetic COF and BSA-based 3DE (Fe3O4@COF@BSA/3DE). We have demonstrated that the Fe3O4@COF@BSA/3DE exhibited excellent chiral recognition to L-Trp as compared to D-Trp. The chiral protein-COF sensing interface was used to determine the concentration of L-Trp in a racemic mixture of D-Trp and L-Trp. This strategy of on-demand fabrication of 3D-printed protein-COF-modified electrodes opens up new approaches for enantiomer recognition.
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