4.5 Article

Molecularly imprinted nanofilms for endotoxin detection using an surface plasmon resonance sensor

Journal

ANALYTICAL BIOCHEMISTRY
Volume 632, Issue -, Pages -

Publisher

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2021.114221

Keywords

Molecular imprinting; Endotoxin; Surface plasmon resonance; Sensor

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A simple, fast, sensitive and selective surface plasmon resonance (SPR) sensor was prepared for endotoxin detection using molecular imprinting method. The sensor showed a limit of detection and quantification of 0.023 and 0.078 ng/mL, respectively, with a response time of approximately 14 minutes. Selectivity studies with cholesterol and hemoglobin, as well as validation studies using limulus amebocyte lysate (LAL), demonstrated the applicability of the SPR sensor for endotoxin detection.
In this study, a simple, fast, sensitive and selective surface plasmon resonance (SPR) sensor was prepared using molecular imprinting method for endotoxin detection. Endotoxin imprinted and non-imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine methyl ester) based nanofilms were synthesized on the SPR chip surfaces using ultraviolet polymerization. Endotoxin imprinted and non-imprinted SPR sensors were characterized by using contact angle, atomic force microscopy and ellipsometry. After characterization studies, kinetic studies was carried out in the concentration range of 0.5-100 ng/mL. The limit of detection and quantification were obtained as 0.023 and 0.078 ng/mL, respectively. The response time for the equilibration, adsorption and regeneration was approximately 14 min. The selectivity studies with cholesterol and hemoglobin of endotoxin imprinted SPR sensor were examined. Validation studies were carried out via limulus amebocyte lysate (LAL) in order to demonstrate the applicability of the SPR sensor.

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