Luminescence lifetime imaging of ultra-long room temperature phosphorescence on a smartphone

Luminescence lifetime imaging plays an important role in distinguishing the luminescence decay rates in timere-solved luminescence imaging. However, traditional imaging instruments used for detecting lifetimes within milliseconds would be time-consuming when imaging ultra-long luminescence lifetimes over subseconds. Herein, we present an accessible and simple optical system for detecting lifetimes of persistent luminescence. A smartphone integrated with a UV LED, a dichroic mirror, and a lens was used for recording the persistent luminescence. With only a few seconds of data acquisition, a luminescence lifetime image could be processed from the video by exponential fitting of the gray level of each pixel to the delay time. Since this approach only requires single excitation, no synchronous control is needed, greatly simplifying the apparatus and saving the cost. The apparatus was successfully used for ultra-long luminescence lifetime imaging of mouse tissue dyed with a persistent luminescence molecule. This miniaturized apparatus exhibits huge potentiality in time-resolved luminescence imaging for luminescence study and biological detection.

Automated summary

Luminescence lifetime imaging is crucial in distinguishing luminescence decay rates. A simple and accessible optical system using a smartphone was presented for detecting lifetimes of persistent luminescence. The system simplifies apparatus, reduces cost, and shows potential for time-resolved luminescence imaging in biological detection.