Journal
ANALYTICA CHIMICA ACTA
Volume 1159, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.aca.2021.338382
Keywords
Pesticide; Aptamers; Fluorescence anisotropy; Isothermal titration calorimetry
Categories
Funding
- ANRT CIFRE
- PolyNatCarnot Institute (Investissements d'Avenir) [ANR-11-CARN-007-01]
Ask authors/readers for more resources
This study aimed to develop fluorescence anisotropy biosensor platforms for pesticide detection, but the isolated DNA aptamers did not bind to the target pesticides. This raises doubts about the effectiveness of aptasensors relying on these particular DNA molecules.
Herein, we originally aimed at developing fluorescence anisotropy biosensor platforms devoted to the homogeneous-phase detection of isocarbophos and phorate pesticides by using previously isolated DNA aptamers. To achieve this, two reporting approaches displaying very high generalizability features were implemented, based on either the complementary strand or the SYBR green intercalator displacement strategies. Unfortunately, none of the transduction methods led to phorate-dependent signals. Only the SYBR green displacement method provided a small output in the presence of isocarbophos, but at an analyte concentration greater than 100 mM. In order to identify the origin of such data, isothermal titration calorimetry (ITC) experiments were subsequently performed. It was shown that aptamers bind neither isocarbophos nor phorate in free solution with the claimed micromolar dissociation constants. This work puts forward some doubts about the previously described aptasensors that rely on the use of these functional DNA molecules. It also highlights the need to carefully investigate the binding capabilities of aptamers after their isolation and to include appropriate control experiments with scrambled or mutated oligonucleotides. (c) 2021 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available