Journal
ANALYTICA CHIMICA ACTA
Volume 1152, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.aca.2021.338269
Keywords
Fluorescent probes; Nanoprobe; Adenosine triphosphate (ATP); Aggregation-induced emission (AIE); Cellular imaging
Categories
Funding
- National Research Foundation (NRF) of Korea (Ministry of Science and ICT) [2019-M3A9H1103783]
- Bio & Medical Technology Development Program of the NRF of Korea (Ministry of Science and ICT) [2018-M3A9H3021707]
- Basic Science Research Program through the NRF of Korea (Ministry of Education) [2018-R1A6A1A03025124, 2018-R1D1A1B07043383]
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This study presents a fluorescent nanoprobe based on aggregation-induced emission luminogen for detecting ATP, showing superior selectivity and sensitivity. Successful in vitro assays and cellular imaging applications have been demonstrated, with potential for further ATP-related studies in various fields.
A turn-on fluorescent nanoprobe (named AAP-1), based on an aggregation-induced emission luminogen (AIEgen), is disclosed for the detection of adenosine triphosphate (ATP), which is an essential element in the biological system. Organic fluorophore (named TPE-TA) consists of tetraphenylethylene (TPE, sensing and signaling moiety) and mono-triamine (TA, sensing moiety), and it forms an aggregated form in aqueous media as a nanoprobe AAP-1. The nanoprobe AAP-1 has multiple electrostatic interactions as well as hydrophobic interactions with ATP, and it displays superior selectivity toward ATP, reliable sensitivity, with a detection limit around 0.275 ppb, and fast responsive (signal within 10 s). Such a fluorescent probe to monitor ATP has been actively pursued throughout fundamental and translational research areas. In vitro assay and a successful cellular ATP imaging application was demonstrated in cancer cells and embryonic stem cells. We expect that our work warrants further ATP-related studies throughout a variety of fields. (c) 2021 Elsevier B.V. All rights reserved.
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