Journal
AGING-US
Volume 13, Issue 7, Pages 10387-10395Publisher
IMPACT JOURNALS LLC
DOI: 10.18632/aging.202800
Keywords
MSC-AS1; miR-142-5p; DDX5; gastric cancer
Categories
Ask authors/readers for more resources
Emerging studies have shown that dysregulated lncRNAs are involved in cancer progression, with MSC-AS1 being overexpressed in gastric cancer cells and enhancing cell growth, inflammatory mediator secretion, and cell cycle progression by modulating DDX5 and inhibiting miR-142-5p expression.
Emerging studies have noted that dysregulated lncRNAs are implicated in cancer progression and tumorigenesis. We first showed that MSC-AS1 was overexpressed in gastric cancer (GC) cells (HGC-27, MKN-45, SGC-7901 and MGC-803 cells) compared with GES cells. We observed that MSC-AS1 was upregulated in GC specimens compared with paired normal specimens. MSC-AS1 increased cell growth and cycle progression. Moreover, the overexpression of MSC-AS1 enhanced the secretion of the inflammatory mediators IL-1 beta, IL-6 and TNF-alpha. We found that the overexpression of MSC-AS1 inhibited the expression of miR-142-5p in HGC-27 cells. We noted that DDK5 was a target gene of miR-142-5p. The overexpression of miR-142-5p suppressed the luciferase activity of wild-type DDX5, but the luciferase activity of the mutant DDX5 was not changed. We showed that miR-142-5p was downregulated in GC specimens compared with paired normal specimens. MSC-AS1 expression was inversely correlated with miR-142-5p expression in GC specimens. MSC-AS1 induced cell growth, cell cycle progression and inflammatory mediator secretion by modulating DDX5. These results showed that MSC-AS1 functions as a key oncogene in the development of GC.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available