Journal
ACS NANO
Volume 15, Issue 4, Pages 6909-6916Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.0c10683
Keywords
gold nanoparticles; DNA; human bone marrow; mRNA; spherical nucleic acid; skeletal stem cell
Categories
Funding
- Biotechnology and Biological Sciences Research Council [BB/P017711/1]
- UK Regenerative Medicine Platform Acellular/Smart Materials -3D Architecture [MR/R015651/1]
- Rosetrees Trust
- Wessex Medical Research
- BBSRC [BB/P017711/1] Funding Source: UKRI
- MRC [MR/R015651/1] Funding Source: UKRI
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Researchers have developed DNA-gold nanoparticles to identify and sort SSCs with specific mRNA signatures, significantly enriching these skeletal stem cells. This method has the potential to enhance our understanding of bone cell biology and translational applications.
Human bone marrow (BM)-derived stromal cells contain a population of skeletal stem cells (SSCs), with the capacity to differentiate along the osteogenic, adipogenic, and chondrogenic lineages, enabling their application to clinical therapies. However, current methods to isolate and enrich SSCs from human tissues remain, at best, challenging in the absence of a specific SSC marker. Unfortunately, none of the current proposed markers alone can isolate a homogeneous cell population with the ability to form bone, cartilage, and adipose tissue in humans. Here, we have designed DNA-gold nanoparticles able to identify and sort SSCs displaying specific mRNA signatures. The current approach demonstrates the significant enrichment attained in the isolation of SSCs, with potential therein to enhance our understanding of bone cell biology and translational applications.
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