Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 13, Issue 19, Pages 22970-22977Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.1c03985
Keywords
liquid chromatography; nanoparticles; mesoporous materials; packing materials; glycan profiling
Funding
- National Natural Science Foundation of China [21834003]
- National Key R&D Program of China [2018YFC0910301]
- Ministry of Science and Technology of China
- [ZYJH004]
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The use of nanomaterials like n-octyl-modified monodispersed dendritic mesoporous silica nanospheres (DMSNs) with small diameter and appropriate pore size has shown great potential in improving separation efficiency in liquid chromatography. The unique morphology of DMSNs allows for highly uniform packing and ultrahigh efficiency, making them a promising candidate for next-generation packing materials. The introduction of DMSNs has opened up new possibilities for achieving ultraefficient liquid chromatographic separations.
Use of smaller particle size of packing materials in liquid chromatography leads to faster separation and higher efficiency. This basic law has driven the evolution of packing materials for several generations. However, the use of nanoscale packing materials has been severely hampered by extremely high back pressure. Here, we report a new possibility of solving this issue via introducing novel nanomaterials with highly favorable structures. n-Octyl-modified monodispersed dendritic mesoporous silica nanospheres (DMSNs) with an unprecedentedly small diameter (ca. 170 nm) and appropriate pore size (5.6 nm) were controllably synthesized and demonstrated to be a practically applicable packing material offering ultrahigh efficiency. The center-radial centrosymmetric mesopore channels significantly improved the permeability of packed capillaries, enabling column packing and capillary electrochromatographic separation on regular instruments. Due to the unique morphology, very tiny particle size, and highly uniform packing, the packed column exhibited ultrahigh efficiency up to 3 500 000 plates/m. Powerful separation capability was demonstrated with glycan profiling of cancerous and normal cells, which revealed that cancerous cells exhibited characteristic N-glycans. Because DMSNs with tunable particle size and mesopores can be controllably prepared, DMSNs hold great potential to be a new record toward the ultimate generation of packing materials for ultraefficient liquid chromatographic separation.
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