Tetraedron minimum, First Reported Member of Hydrodictyaceae to Accumulate Secondary Carotenoids

We isolated a novel strain of the microalga Tetraedron minimum in Iceland from a terrestrial habitat. During long-term cultivation, a dish culture turned orange, indicating the presence of secondary pigments. Thus, we characterized T. minimum for growth and possible carotenoid production in different inorganic media. In a lab-scale photobioreactor, we confirmed that nitrogen starvation in combination with salt stress triggered a secondary carotenoid accumulation. The development of the pigment composition and the antioxidant capacity of the extracts was analyzed throughout the cultivations. The final secondary carotenoid composition was, on average, 61.1% astaxanthin and 38.9% adonixanthin. Moreover, the cells accumulated approx. 83.1% unsaturated fatty acids. This work presents the first report of the formation of secondary carotenoids within the family Hydrodictyaceae (Sphaeropleales, Chlorophyta).

Automated summary

This study isolated a novel strain of microalga Tetraedron minimum in Iceland and characterized its growth and carotenoid production in different media, revealing nitrogen starvation combined with salt stress triggers secondary carotenoid accumulation. The final composition included primarily astaxanthin and adonixanthin, alongside high levels of unsaturated fatty acids, marking the first report of secondary carotenoid formation in the family Hydrodictyaceae.