Size Selective Harvesting Does Not Result in Reproductive Isolation among Experimental Lines of Zebrafish, Danio rerio: Implications for Managing Harvest-Induced Evolution

Simple Summary Mortality in fish populations is commonly size-selective. In fisheries, larger fish are preferentially caught while natural predators preferentially consume smaller fish. Removal of certain sized fish from populations and elevated fishing mortality constitute a selection pressure which may change life-history, behaviour and reduce adult body-size. Because behaviour and body-size are related and influence mating preferences and reproductive output, size-selective mortality may favour subpopulations that less readily mate with each other. Our aim is to test this possibility using three experimental lines of zebrafish (Danio rerio) generated in laboratory by removing large-sized, small-sized and random-sized fish for five generations. We tested mating preferences among males and females and tested if they spawned together. We found males and females of all subpopulations to reproduce among themselves. Females generally preferred large-sized males. Females of all lines spawned with males, and males of all lines fertilised eggs of females independent of the subpopulation origin. Our study shows that size-selective mortality typical of fisheries or in populations facing heavy predation does not result in evolution of reproductive barriers. Thus, when populations adapted to fishing pressure come in contact with populations unexposed to such pressures, interbreeding may happen thereby helping exploited populations recover from harvest-induced evolution. Size-selective mortality is common in fish stocks. Positive size-selection happens in fisheries where larger size classes are preferentially targeted while gape-limited natural predation may cause negative size-selection for smaller size classes. As body size and correlated behavioural traits are sexually selected, harvest-induced trait changes may promote prezygotic reproductive barriers among selection lines experiencing differential size-selective mortality. To investigate this, we used three experimental lines of zebrafish (Danio rerio) exposed to positive (large-harvested), negative (small-harvested) and random (control line) size-selective mortality for five generations. We tested prezygotic preferences through choice tests and spawning trials. In the preference tests without controlling for body size, we found that females of all lines preferred males of the generally larger small-harvested line. When the body size of stimulus fish was statistically controlled, this preference disappeared and a weak evidence of line-assortative preference emerged, but only among large-harvested line fish. In subsequent spawning trials, we did not find evidence for line-assortative reproductive allocation in any of the lines. Our study suggests that size-selection due to fisheries or natural predation does not result in reproductive isolation. Gene flow between wild-populations and populations adapted to size-selected mortality may happen during secondary contact which can speed up trait recovery.

Automated summary

The study shows that size-selective mortality, typical in fisheries or populations facing heavy predation, does not lead to evolution of reproductive barriers. Gene flow between populations adapted to fishing pressure and those unexposed to such pressures can occur during secondary contact, aiding exploited populations in recovering from harvest-induced evolution.