Sexual excitation induces courtship ultrasonic vocalizations and cataplexy-like behavior in orexin neuron-ablated male mice

Cataplexy is triggered by laughter in humans and palatable food in mice. To further evaluate mice's cataplexy, we examined courtship behavior in orexin neuron-ablated mice (ORX-AB), one of the animal models of narcolepsy/cataplexy. Wild-type female mice were placed into the home cage of male ORX-AB and cataplexy-like behavior was observed along with ultrasonic vocalizations (USVs), also known as the love song. ORX-AB with a female encounter showed cataplexy-like behavior both during the dark and light periods, whereas ORX-AB with chocolate predominantly showed it during the dark period. During the light period observation, more than 85% of cataplexy-like bouts were preceded by USVs. A strong positive correlation was observed between the number of USVs and cataplexy-like bouts. Cataplexy-like behavior in narcoleptic mice is a good behavioral measure to study the brain mechanisms behind positive emotion because they can be induced by different kinds of positive stimuli, including chocolate and female courtship. Kuwaki and Kanno examine courtship behavior in orexin neuron-ablated mice (ORX-AB), which are a model of narcolepsy/cataplexy. They find that ORX-AB mice showed cataplexy-like behavior during both dark and light periods in response to a female encounter, however this behavior was predominantly present during dark periods when exposed to chocolate. Studying cataplexy-like behavior in narcoleptic mice is useful for understanding mechanisms behind positive emotions, such as those associated with chocolate and courtship.

Automated summary

The study examined courtship behavior in orexin neuron-ablated mice (ORX-AB), a model of narcolepsy/cataplexy, and found that these mice displayed cataplexy-like behavior in response to female encounter, with a predominant presence during dark periods when exposed to chocolate. Studying cataplexy-like behavior in narcoleptic mice is useful for understanding mechanisms behind positive emotions, such as those associated with chocolate and courtship.