Energetics of mesoscale cell turbulence in two-dimensional monolayers

Investigation of energy mechanisms at the collective cell scale is a challenge for understanding various biological processes, such as embryonic development and tumor metastasis. Here we investigate the energetics of self-sustained mesoscale turbulence in confluent two-dimensional (2D) cell monolayers. We find that the kinetic energy and enstrophy of collective cell flows in both epithelial and non-epithelial cell monolayers collapse to a family of probability density functions, which follow the q-Gaussian distribution rather than the Maxwell-Boltzmann distribution. The enstrophy scales linearly with the kinetic energy as the monolayer matures. The energy spectra exhibit a power-decaying law at large wavenumbers, with a scaling exponent markedly different from that in the classical 2D Kolmogorov-Kraichnan turbulence. These energetic features are demonstrated to be common for all cell types on various substrates with a wide range of stiffness. This study provides unique clues to understand active natures of cell population and tissues. Understanding the mechanisms underlying collective motion in cells is central to understanding tissue development as well as the emergence of several pathologies. Here, the authors study the mesoscale turbulence of confluent cell monolayers through a combined experimental and numerical approach, finding that energetic statistics are independent of cell types and substrate stiffness.

Automated summary

The energetic characteristics of collective cell flows in confluent cell monolayers follow a q-Gaussian distribution and exhibit power-decaying energy spectra. These features are independent of cell types and substrate stiffness, providing unique clues to understanding the active nature of cell populations and tissues.