Journal
BIOMEDICINES
Volume 9, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/biomedicines9020199
Keywords
periodontitis; periodontal bone-loss; decitabine; Kruppel-like transcription factor-2; interleukin-10; transforming growth factor beta-1
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Funding
- National Institutes of Health, NIDCR [DE017384]
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This study demonstrated that Decitabine inhibits bone loss and osteoclast differentiation in experimental periodontitis, while upregulating anti-inflammatory cytokines through a KLF2-dependent mechanism. This suggests that DNA methyltransferase inhibitors could be a potential novel therapy for periodontitis.
DNA methylation controls several inflammatory genes affecting bone homeostasis. Hitherto, inhibition of DNA methylation in vivo in the context of periodontitis and osteoclastogenesis has not been attempted. Ligature-induced periodontitis in C57BL/6J mice was induced by placing ligature for five days with Decitabine (5-aza-2'-deoxycytidine) (1 mg/kg/day) or vehicle treatment. We evaluated bone resorption, osteoclast differentiation by tartrate-resistant acid phosphatase (TRAP) and mRNA expression of anti-inflammatory molecules using cluster differentiation 14 positive (CD14(+)) monocytes from human peripheral blood. Our data showed that decitabine inhibited bone loss and osteoclast differentiation experimental periodontitis, and suppressed osteoclast CD14(+) human monocytes; and conversely, that it increased bone mineralization in osteoblastic cell line MC3T3-E1 in a concentration-dependent manner. In addition to increasing IL10 (interleukin-10), TGFB (transforming growth factor beta-1) in CD14(+) monocytes, decitabine upregulated KLF2 (Kruppel-like factor-2) expression. Overexpression of KLF2 protein enhanced the transcription of IL10 and TGFB. On the contrary, site-directed mutagenesis of KLF2 binding site in IL10 and TFGB abrogated luciferase activity in HEK293T cells. Decitabine reduces bone loss in a mouse model of periodontitis by inhibiting osteoclastogenesis through the upregulation of anti-inflammatory cytokines via KLF2 dependent mechanisms. DNA methyltransferase inhibitors merit further investigation as a possible novel therapy for periodontitis.
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