Journal
AQUACULTURE REPORTS
Volume 19, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.aqrep.2020.100573
Keywords
Oreochromis niloticus; HMGB1; monocytes; macrophages; Immune response; Bactericidal activity
Categories
Funding
- National Natural Science Foundation of China [31302226, 31572651, 32073006, 32002426]
- National Key R & D Program of China [2018YFD0900501]
- Technology Planning Project of Guangdong Province of China [2015A020209181, 2017A030307033]
- Fund of Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang) [ZJW-2019-06]
- Special foundation for achieving the first class of Guangdong Province [231419013]
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The OnHMGB1 gene was identified and characterized in Nile tilapia, and plays a crucial role in immune response, especially against pathogen infection.
High-mobility group box1 (HMGB1) is a non-histone chromosome protein that not only participates in nuclear formation and transcriptional regulation of, but also acts as a pro-inflammatory factor. In this study, a HMGB1 gene (OnHMGB1) was identified and characterized from Nile tilapia (Oreochromis niloticus). The open reading frame of OnHMGB1 sequence was 621 bp, encoding a 207 amino acids peptide. The deduced OnHMGB1 protein contained two HMG boxes and an acidic tail, which shared high identities to other reported HMGB1. OnHMGB1 was widely distributed in many tissues of healthy tilapia and was significantly up-regulated in spleen and head kidney after Streptococcus agalactiae poly I:C stimulation in vivo. Moreover, the recombinant protein of OnHMGB1 (rOnHMGB1) could bind to DNA in a dose-dependent manner, promote inflammatory factor expression and bactericidal activity of monocytes/macrophages in vitro as well. Taken together, these results indicate that OnHMGB1 plays roles in immune response of Nile tilapia against pathogen infection.
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