Advances in Mutation Detection Using Loop-Mediated Isothermal Amplification

Detection of mutations and single-nucleotide polymorphisms is highly important for diagnostic applications. Loop-mediated isothermal amplification (LAMP) is a powerful technique for the rapid and sensitive detection of nucleic acids. However, LAMP traditionally does not possess the ability to resolve single-nucleotide differences within the target sequence. Because of its speed and isothermal nature, LAMP is ideally suited for point-of-care applications in resource-limited settings. Recently, different approaches have been developed and applied to enable single-nucleotide differentiation within target sequences. This Mini-Review highlights advancements in mutation detection using LAMP. Methods involving primer design and modification to enable sequence differentiation are discussed. In addition, the development of probe-based detection methods for mutation detection are also covered.

Automated summary

Detection of mutations and single-nucleotide polymorphisms is crucial for diagnostic applications, and Loop-mediated isothermal amplification (LAMP) is a powerful tool for this purpose. However, traditional LAMP lacks the ability to distinguish single-nucleotide differences within target sequences. Various approaches have been developed to address this limitation, including primer design modifications and probe-based detection methods.