Genotyping-by-Sequencing in Vigna unguiculata Landraces and Its Utility for Assessing Taxonomic Relationships

Genotyping by sequencing (GBS) was used to analyze relationships among cowpea and asparagus bean landraces from southern Italy and to assess the utility of this technology to study taxonomy in a wider panel, including V. unguiculata cultigroups, subspecies, and other Vigna species. The analysis of SNPs derived from GBS highlighted that, among the cowpea landraces, the African samples were separated from the other material, while, for the Italian landraces, a certain clustering depending on seed color/pattern was observed in the dendrogram. When examining the V. unguiculata species complex, a clear separation between the two groups of wild subspecies, i.e., the allogamous wild perennials and the perennial out/inbreds, could be observed, the former representing the more ancestral wild progenitors of V. unguiculata. The species V. vexillata appeared more closely related to V. unguiculata than to the other Vigna species analyzed.

Automated summary

Genotyping by sequencing (GBS) was used to analyze the relationships among cowpea and asparagus bean landraces from southern Italy, showing clear separation between African samples and others, as well as clustering based on seed color/pattern in Italian landraces. When examining the V. unguiculata species complex, a separation between wild subspecies groups was observed, with V. vexillata appearing more closely related to V. unguiculata than to other Vigna species.