Response of Three Greek Populations of Aegilops triuncialis (Crop Wild Relative) to Serpentine Soil

A common garden experiment was established to investigate the effects of serpentine soil on the photosynthetic and biochemical traits of plants from three Greek populations of Aegilops triuncialis. We measured photosynthetic and chlorophyll fluorescence parameters, proline content, and nutrient uptake of the above plants growing in serpentine and non-serpentine soil. The photochemical activity of PSII was inhibited in plants growing in the serpentine soil regardless of the population; however, this inhibition was lower in the Aetolia-Acarnania population. The uptake and the allocation of Ni, as well as that of some other essential nutrient elements (Ca, Mg, Fe, Mn), to upper parts were decreased with the lower decrease recorded in the Aetolia-Acarnania population. Our results showed that excess Ni significantly increased the synthesis of proline, an antioxidant compound that plays an important role in the protection against oxidative stress. We conclude that the reduction in the photosynthetic performance is most probably due to reduced nutrient supply to the upper plant parts. Moreover, nickel accumulation in the roots recorded in plants from all three populations seems to be a mechanism to alleviate the detrimental effects of the serpentine soil stress. In addition, our data suggest that the population from Aetolia-Acarnania could be categorized among the nickel excluders.

Automated summary

The study investigated the effects of serpentine soil on plants from three Greek populations of Aegilops triuncialis, revealing inhibited photochemical activity of PSII, reduced nutrient uptake and allocation to upper plant parts, increased proline synthesis due to excess nickel, and a possible mechanism of nickel accumulation in roots to mitigate the negative effects of serpentine soil stress. The population from Aetolia-Acarnania exhibited lower levels of inhibition and reduction, suggesting a potential categorization as nickel excluders.