Journal
PLANTS-BASEL
Volume 10, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/plants10020373
Keywords
soybean; transposable element; mutation breeding; gamma ray; TE-TRAP
Categories
Funding
- Radiation Technology R&D Program through the National Research Foundation of Korea - Ministry of Science and ICT [NRF-2017M2A2A6A05018538]
- National Research Foundation of Korea [2017M2A2A6A05018538] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The study used TE-TRAP technique combined with sensitive MITEs to investigate the mobility of TEs in gamma-irradiated soybean mutant pool, revealing that MITE markers showed more dynamic and active mobility levels compared to the PONG marker.
Transposable elements (TEs)-major components of eukaryotic genomes-have the ability to change location within a genome. Because of their mobility, TEs are important for genome diversification and evolution. Here, a simple rapid method, using the consensus terminal inverted repeat sequences of PONG, miniature inverted-repeat transposable element (MITE)-Tourist (M-t) and MITE-Stowaway (M-s) as target region amplification polymorphism (TE-TRAP) markers, was employed to investigate the mobility of TEs in a gamma-irradiated soybean mutant pool. Among the different TE-TRAP primer combinations, the average polymorphism level and polymorphism information content value were 57.98% and 0.14, respectively. Only the PONG sequence separated the mutant population into three major groups. The inter-mutant population variance, determined using the PONG marker (3.151 and 29%) was greater than that of the M-t (2.209 and 20%) and M-s (2.766 and 18%) markers, whereas the reverse was true for the intra-mutant population variations, with M-t and M-s values, being 15.151 (82%) and 8.895 (80%), respectively, compared with the PONG marker (7.646 and 71%). Thus, the MITE markers revealed more dynamic and active mobility levels than the PONG marker in gamma-ray irradiated soybean mutant lines. The TE-TRAP technique associated with sensitive MITEs is useful for investigating genetic diversity and TE mobilization, providing tools for mutant selection in soybean mutation breeding.
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