Journal
FOODS
Volume 10, Issue 3, Pages -Publisher
MDPI
DOI: 10.3390/foods10030541
Keywords
phenolic compounds; Vitis vinifera; ripening; grapes; anti-inflammatory; antioxidant activity
Categories
Funding
- Bordeaux Metabolome Facility
- MetaboHUB [ANR-11-INBS-0010]
Ask authors/readers for more resources
The study characterized the polyphenolic composition of grape skins from Merlot, Tannat, and Syrah varieties using UPLC-QqQ-MS. Changes in polyphenol content during ripening were observed, with an increase in anthocyanins and stilbenes, and a decrease in flavan-3-ols and procyanidins. The skin extracts showed strong antioxidant and anti-inflammatory properties, with significant inhibition of NO and ROS production in stimulated macrophages.
(1) Background: Both sensory quality and healthy attributes of Vitis vinifera grapes used for winemaking are closely related with the polyphenolic composition of their skins. (2) Methods: In this study, the polyphenolic characterization (flavan-3-ols, procyanidins, flavonols, stilbenes, anthocyanins) was investigated by ultra performance liquid chromatography coupled to a triple quadrupole mass spectrometer (UPLC-QqQ-MS). Skins from Vitis vinifera Merlot, Tannat, and Syrah red grape varieties cultivated in the south of France at different stages of ripening in 2018 were used. The anti-inflammatory and the antioxidant potential of the extracts were evaluated by the measure of nitric oxide (NO) and the intracellular reactive oxygen species production (ROS) in lipopolysaccharide (LPS)-stimulated macrophages. (3) Results: 41 polyphenols were quantified in all samples. Generally, the flavan-3-ol and procyanidin content decreased during ripening whereas the anthocyanins and stilbenes increased. In addition, as a novelty of this work, a wide identification and characterization of monomeric and oligomeric stilbenes was assessed by using authentic standards isolated in our laboratory, some of them (parthenocissin A and miyabenol C) reported for the first time in Merlot, Tannat and Syrah cultivars. The before-veraison skin extracts of all studied varieties, exhibited higher NO and ROS productions inhibition (>50%) proving both antioxidant and anti-inflammatory properties.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available