Journal
PATHOGENS
Volume 10, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/pathogens10020202
Keywords
PRRSV; tea polyphenol; antiviral; cytokine
Categories
Funding
- Science and Technology Planning Project for Guangzhou [201804020039]
- Natural Science Foundation of Guangdong Province [2019B1515210024]
- National Natural Science Foundation of China [31872329, 32072695]
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TPP effectively inhibits PRRSV infection in Marc-145 cells by suppressing viral attachment, internalization, replication, and release. It also prevents the entry of p65 into the nucleus, suppressing the activation of the NF-kappa B signaling pathway and the expression of inflammatory cytokines. Additionally, TPP limits the synthesis of viral non-structural protein 2, which may contribute to the inhibition of viral RNA replication.
Neither inactivated nor attenuated vaccines can effectively prevent and control the infection and spread of porcine reproductive and respiratory syndrome virus (PRRSV). Therefore, it is necessary to broaden new horizons and to conceive effective preventive strategies. The main components of Tea polyphenol (TPP) are catechins and their derivatives. TPP has many physiological activities and has certain antiviral and antifungal effects. However, whether TPP shows anti-PRRSV activity remains unclear. We found that TPP effectively inhibited PRRSV infection in Marc-145 cells by suppressing the stages of viral attachment, internalization, replication, and release. TPP exhibited a potent anti-PRRSV effect regardless of pre-treatment or post-treatment. In addition, we demonstrated that TPP restrained PRRSV-induced p65 entry into the nucleus to suppress the activation of the NF-kappa B signaling pathway, which ultimately leads to the inhibition of the expression of inflammatory cytokines. Furthermore, TPP limited the synthesis of viral non-structural protein 2 (nsp2), the core component of viral replication transcription complexes, which may contribute to the inhibition of viral RNA replication. TPP has the potential to develop into an effective antiviral agent for PRRSV prevention and control in the future.
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