Journal
MICROORGANISMS
Volume 9, Issue 3, Pages -Publisher
MDPI
DOI: 10.3390/microorganisms9030524
Keywords
bacteriophage; viral metagenomics; phageome; viral extraction
Categories
Funding
- Science Foundation Ireland [12/RC/2273_P2]
- Science Foundation Ireland (SFI) [12/RC/2273_P2] Funding Source: Science Foundation Ireland (SFI)
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The human gut is colonized by a diverse range of microbes, including viruses, which have recently attracted more attention in research. Different viral extraction protocols used in studies can introduce biases affecting overall conclusions, highlighting the need for clearly outlining protocol variations and perhaps establishing an unbiased and standardized method for examining the viral component of the human microbiome.
The human gut is colonised by a vast array of microbes that include bacteria, viruses, fungi, and archaea. While interest in these microbial entities has largely focused on the bacterial constituents, recently the viral component has attracted more attention. Metagenomic advances, compared to classical isolation procedures, have greatly enhanced our understanding of the composition, diversity, and function of viruses in the human microbiome (virome). We highlight that viral extraction methodologies are crucial in terms of identifying and characterising communities of viruses infecting eukaryotes and bacteria. Different viral extraction protocols, including those used in some of the most significant human virome publications to date, have introduced biases affecting their a overall conclusions. It is important that protocol variations should be clearly highlighted across studies, with the ultimate goal of identifying and acknowledging biases associated with different protocols and, perhaps, the generation of an unbiased and standardised method for examining this portion of the human microbiome.
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