Journal
MICROORGANISMS
Volume 9, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/microorganisms9020283
Keywords
HBV genotypes; HBcAg expression; encapsidation
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Funding
- European Regional Development Fund [2013/0053/2DP/2.1.1.1.0/13/APIA/VIAA/006]
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The core proteins (HBc) of hepatitis B virus (HBV) genotypes A, B, C, D, E, F, and G were cloned and expressed in Escherichia coli (E. coli), and the VLPs formed by HBc were purified with various methods. The best VLP yield was observed for HBV genotypes D and G, with successful packaging of RNA demonstrated.
The core proteins (HBc) of the hepatitis B virus (HBV) genotypes A, B, C, D, E, F, and G were cloned and expressed in Escherichia coli (E. coli), and HBc-formed virus-like particles (VLPs) were purified with ammonium sulfate precipitation, gel filtration, and ion exchange chromatography (IEX). The best VLP yield was found for the HBc of the HBV genotypes D and G. For the HBc of the HBV genotypes D, F, and G, the possibility of dissociation and reassociation maintaining the native HBc structure was demonstrated. Single-stranded (ss) and double-stranded (ds) ribonucleic acid (RNA) was successfully packed into HBc VLPs for the HBV genotypes D and G.
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