Journal
MICROORGANISMS
Volume 9, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/microorganisms9020447
Keywords
Pseudomonas aeruginosa; Glucose; periplasmic binding protein; two-component system; transcriptional regulation
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Funding
- National Natural Science Foundation (NSFC) [31870127, 81861138047]
- Ministry of Science and Technology (MOST) of China [2016YFA0501503, 2019ZX09721001-004-003]
- Science and Technology Commission of Shanghai Municipality [19JC1416400]
- State Key Laboratory of Drug Research [SIMM2003ZZ-03]
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Research shows that the periplasmic binding protein GltB plays a crucial role in activating GtrS-GltR in response to glucose in P. aeruginosa and together they regulate the virulence of P. aeruginosa in response to glucose. Furthermore, the activation of GltB is closely related to the virulence of P. aeruginosa.
A two-component system GtrS-GltR is required for glucose transport activity in P. aeruginosa and plays a key role during P. aeruginosa-host interactions. However, the mechanism of action of GtrS-GltR has not been definitively established. Here, we show that gltB, which encodes a periplasmic glucose binding protein, is essential for the glucose-induced activation of GtrS-GltR in P. aeruginosa. We determined that GltB is capable of binding to membrane regulatory proteins including GtrS, the sensor kinase of the GtrS-GltR TCS. We observed that alanine substitution of glucose-binding residues abolishes the ability of GltB to promote the activation of GtrS-GltR. Importantly, like the gtrS deletion mutant, gltB deletion mutant showed attenuated virulence in both Drosophila melanogaster and mouse models of infection. In addition, using CHIP-seq experiments, we showed that the promoter of gltB is the major in vivo target of GltR. Collectively, these data suggest that periplasmic binding protein GltB and GtrS-GltR TCS form a complex regulatory circuit that regulates the virulence of P. aeruginosa in response to glucose.
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