Enzyme-Assisted Release of Antioxidant Peptides from Porphyra dioica Conchocelis

The conchocelis life cycle stage of P. dioica represents an unexplored source of bioactive compounds. The aim of this study was to generate and characterise, for the first time, hydrolysates of conchocelis using a specific combination of proteases (Prolyve (R) and Flavourzyme (R)). Hydrolysate molecular mass distribution and free amino acid contents were assessed, and the antioxidant activity was determined using a range of in vitro assays. The protein content and the total amino acid profiles of conchocelis were also studied. Conchocelis contained similar to 25% of protein (dry weight basis) and had a complete profile of essential amino acids. Direct sequential enzymatic treatment modified the profile of the generated compounds, increasing the amount of low molecular weight peptides (<1 kDa). There was a significant improvement in the antioxidant activity of the hydrolysates compared with the control (up to 2.5-fold), indicating their potential as a novel source of antioxidant ingredients.

Automated summary

The conchocelis life cycle stage of P. dioica is an unexplored source of bioactive compounds. A specific combination of proteases was used to generate and characterize hydrolysates of conchocelis for the first time, showing improved antioxidant activity and potential as a novel source of antioxidant ingredients.