Journal
BIOMOLECULES
Volume 11, Issue 3, Pages -Publisher
MDPI
DOI: 10.3390/biom11030345
Keywords
abscisic acid; microbial metabolite; dehydrovomifoliol; NMR spectrometry; phytohormones; rhizosphere; Rhodococcus
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Funding
- Russian Science Foundation [17-14-01363, 19-16-00097]
- Ministry of Science and Higher Education of the Russian Federation [075-15-2019-1881]
- Russian Science Foundation [17-14-01363, 19-16-00097] Funding Source: Russian Science Foundation
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The research revealed that the strain Rhodococcus sp. P1Y can degrade ABA and produce dehydrovomifoliol. The generation of this metabolite is achieved by gradually shortening the acyl part of the ABA molecule.
The phytohormone abscisic acid (ABA) plays an important role in plant growth and in response to abiotic stress factors. At the same time, its accumulation in soil can negatively affect seed germination, inhibit root growth and increase plant sensitivity to pathogens. ABA is an inert compound resistant to spontaneous hydrolysis and its biological transformation is scarcely understood. Recently, the strain Rhodococcus sp. P1Y was described as a rhizosphere bacterium assimilating ABA as a sole carbon source in batch culture and affecting ABA concentrations in plant roots. In this work, the intermediate product of ABA decomposition by this bacterium was isolated and purified by preparative HPLC techniques. Proof that this compound belongs to ABA derivatives was carried out by measuring the molar radioactivity of the conversion products of this phytohormone labeled with tritium. The chemical structure of this compound was determined by instrumental techniques including high-resolution mass spectrometry, NMR spectrometry, FTIR and UV spectroscopies. As a result, the metabolite was identified as (4RS)-4-hydroxy-3,5,5-trimethyl-4-[(E)-3-oxobut-1-enyl]cyclohex-2-en-1-one (dehydrovomifoliol). Based on the data obtained, it was concluded that the pathway of bacterial degradation and assimilation of ABA begins with a gradual shortening of the acyl part of the molecule.
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