Journal
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
Volume 9, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fcell.2021.609311
Keywords
CLTC; spindle assembly; chromosome congression; oocyte; early embryo development
Categories
Funding
- NSF of China [31960158, 31871508, 31671560, 31900596]
- Major Projects of Natural Science Foundation of Inner Mongolia Autonomous Region [2019ZD09]
- Natural Science Foundation of Inner Mongolia Autonomous Region [2019BS03027]
- Key Technology Research Plan Project of Inner Mongolia Autonomous Region [2019GG103]
- Science and Technology Major Project of Inner Mongolia Autonomous Region of China [2020ZD0008]
Ask authors/readers for more resources
The study investigated the expression profiles and functional roles of CLTC in sheep oocytes and found that CLTC plays crucial regulatory roles in sheep oocyte maturation and early embryo development, especially in spindle dynamics and early embryo development. The results indicate that CLTC is essential for spindle formation and chromosomal alignment, as well as for proper early embryo development.
As a major protein of the polyhedral coat of coated pits and vesicles, clathrin molecules have been shown to play a stabilization role for kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridges. Clathrin heavy chain 1 (CLTC), the basic subunit of the clathrin coat, plays vital roles in both spindle assembly and chromosome congression during somatic-cell mitosis. However, its function in oocyte meiotic maturation and early embryo development in mammals, especially in domesticated animals, has not been fully investigated. In this study, the expression profiles and functional roles of CLTC in sheep oocytes were investigated. Our results showed that the expression of CLTC was maintained at a high level from the germinal vesicle (GV) stage to metaphase II stage and that CLTC was distributed diffusely in the cytoplasm of cells at interphase, from the GV stage to the blastocyst stage. After GV breakdown (GVBD), CLTC co-localized with beta-tubulin during metaphase. Oocyte treatments with taxol, nocodazole, or cold did not affect CLTC expression levels but led to disorders of its distribution. Functional impairment of CLTC by specific morpholino injections in GV-stage oocytes led to disruptions in spindle assembly and chromosomal alignment, accompanied by impaired first polar body (PB1) emissions. In addition, knockdown of CLTC before parthenogenetic activation disrupted spindle formation and impaired early embryo development. Taken together, the results demonstrate that CLTC plays a vital role in sheep oocyte maturation via the regulation of spindle dynamics and an essential role during early embryo development.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available