Journal
JOURNAL OF ADVANCED RESEARCH
Volume 31, Issue -, Pages 155-163Publisher
ELSEVIER
DOI: 10.1016/j.jare.2021.01.010
Keywords
M1 macrophage; Photothermal treatment; Photothermal effect; Live cell vector; Cancer therapy; Medical optics and biotechnology
Categories
Funding
- Clinical Trial Center of Korea University Anam Hospital [I1500931]
- Korea Health Technology RAMP
- D Project through the Korea Health Industry Development Institute (KHIDI) by the Ministry of Health Welfare [HI14C0748]
- Basic Science Research Program through the National Research Foundation of Korea - Ministry of Education [NRF-2016R1D1A1A02937362, NRF-2018R1D1A1A09083263, NRF-2019R1A2C4004804, NRF-2019H1A2A1076334]
- National Eye Institute [R01EY030569]
- Institute of Information and Communications Technology Planning and Evaluation (IITP
- MSIT) [2020-0-00997]
- Korea University
- National Research Foundation of Korea [2019H1A2A1076334] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Activated M1 macrophages demonstrated higher uptake capacity and active infiltration towards NPs compared to non-activated M Phi macrophages. Using M1 as live vector led to a more rapid reduction in tumor size, indicating higher efficacy of PTT with NPs-loaded M1.
Introduction: To enhance photothermal treatment (PTT) efficiency, a delivery method that uses cell vector for nanoparticles (NPs) delivery has drawn attention and studied widely in recent years. Objectives: In this study, we demonstrated the feasibility of M1 activated macrophage as a live vector for delivering NPs and investigated the effect of NPs loaded M1 stimulated by Lipopolysaccharide on PTT efficiency in vivo. Methods: M1 was used as a live vector for delivering NPs and further to investigate the effect of NPs loaded M1 on PTT efficiency. Non-activated macrophage (M Phi) was stimulated by lipopolysaccharide (LPS) into M1 and assessed for tumor cell phagocytic capacity towards NPs Results: We found M1 exhibited a 20-fold higher uptake capacity of NPs per cell volume and 2.9-fold more active infiltration into the tumor site, compared with non-activated macrophage M Phi. We injected M1 cells peritumorally and observed that these cells penetrated into the tumor mass within 12 h. Then, we conducted PTT using irradiation of a near-infrared laser for 1 min at 1 W/cm(2). As a result, we confirmed that using M1 as an active live vector led to a more rapid reduction in tumor size within 1 day indicating that the efficacy of PTT with NPs-loaded M1 is higher than that with NPs-loaded M Phi. Conclusion: Our study demonstrated the potential role of M1 as a live vector for enhancing the feasibility of PTT in cancer treatment. (C) 2021 The Authors. Published by Elsevier B.V. on behalf of Cairo University.
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