Journal
FRONTIERS IN GENETICS
Volume 11, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fgene.2020.608178
Keywords
polycystic ovary syndrome; follicle fluid; exosomes; long non-coding RNAs; bioinformatics
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Funding
- National Natural Science Foundation of China [82001512]
- Jiangsu Province Maternal and Child Health Research Project [F201945]
- Science and Technology Development Plan Project of Jilin Province [20200404169, 20180101140]
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This study conducted high-throughput lncRNA sequencing of follicular fluid exosomes in PCOS and non-PCOS patients, identifying significant upregulation of hormones like estrogen, testosterone, and luteinizing hormone in PCOS patients. Functional analysis indicated potential involvement of lncRNAs in the pathogenesis of PCOS.
Polycystic ovary syndrome (PCOS) is a common reproductive endocrine disease characterized by persistent anovulation and hyperandrogenism, affecting approximately 8-10% of women of childbearing age and occupying an important position in the etiology of infertility. There is increasing evidence that long non-coding RNAs (lncRNAs) are involved in the development of PCOS, but the potential regulatory mechanism is still unclear. This study performed high-throughput lncRNA sequencing of follicular fluid exosomes in non-PCOS infertility patients and PCOS infertility patients. The sequencing results led to the identification of 1,253 upregulated and 613 downregulated lncRNAs from a total of 1,866 detected candidates. There was no significant difference between the PCOS patients and non-PCOS patients in body mass index (BMI) or the fasting blood glucose (FBG) level. However, luteinizing hormone (LH), estradiol (E2), testosterone (T), serum prolactin (PRL), and anti-Mullerian hormone (AMH) levels were clearly upregulated in PCOS patients compared to those in non-PCOS patients. There was also an increase in LH/FSH (>2) in the PCOS patients. Functional analysis showed pathways related to endocytosis, the Hippo, the MAPK, and HTLV-1 infection. These results suggest that lncRNAs may play an important role in the pathogenesis of PCOS and may be potential targets for the diagnosis and treatment of PCOS.
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