Journal
ENVIRONMENTAL SCIENCE & TECHNOLOGY
Volume 51, Issue 1, Pages 291-300Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.est.6b04885
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Funding
- National Science Foundation for Distinguished Young Scholars of China [51225802]
- National Natural Science Foundation of China [31500084]
- State Key Laboratory of Urban Water Resource and Environment of Harbin Institute of Technology [2016DX03]
- Hundred Talents Program of the Chinese Academy of Sciences [29BR2013001]
- Key Deployment Project of Chinese Academy of Sciences [ZDRW-ZS-2016-5-5]
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Haloaromatic antimicrobial triclocarban (3,4,4'-trichlorocarbanilide, TCC) is a refractory contaminant which is frequently deteded in various aquatic and sediment environments globally. However, few TCC-degrading communities or pure cultures have been documented, and functional enzymes involved in TCC biodegradation hitherto have-not yet been characterized. In this study, a bacterial strain, Ochrobactrum sp. TCC-2, capable of degrading TCC under both aerobic and anaerobic conditions was isolated from a sediment sample. A novel amidase gene (tccA); responsible for the hydrolysis of the two amide bonds of TCC and its dehalogenated congeners 4,4'-dichlorocarbanilide (DCC) and carbanilide (NCC) to more biodegradable chloroaniline or aniline products; was cloned and characterized. TccA shares low amino acid sequence identity (27 to 38%) with other biochemically characterized amidases and contains the conserved catalytic triad (Ser-Ser-Lys) of the arnidase signature enzyme family. TccA was stable over a pH range of 5.0 to 10.0 and at temperatures lower than 60 degrees C, and it was constitutively expressed in strain TCC-2. In contrast to the halogenated TCC and DCC, the nonchlorinated NCC was the preferred substrate for TccA. TccA also had hydrolysis activity to a :broad spectrum of amide bonds in herbicides, insecticides, and chemical intermediates. The constitutive expression and broad substrate spectrum of TccA suggested strain TCC-2 may be potentially useful for bioremediation applications.
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