Journal
MATERIALS & DESIGN
Volume 200, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.matdes.2021.109468
Keywords
Thiolated icariin; Controlled release; High loading capacity; Osteoporotic bone regeneration; BCP ceramic
Categories
Funding
- National Natural Science Foundation of China [51873116, 31971283]
- Provincial key Research and Development Programs of Science and Technology of Sichuan [2020YFS0407]
- China Postdoctoral Innovation Talent Support program [BX20180204]
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The use of Ica-SH/BCP scaffolds resulted in lower drug release rate, promoting osteoblastic cell proliferation, migration, and differentiation, as well as up-regulating angiogenic gene expression in vitro. In an osteoporotic animal model, Ica-SH/BCP scaffolds were shown to enhance bone regeneration.
The regeneration of bone defects in osteoporotic patients presents a big challenge. Icariin (Ica), which is the main active component of traditional Chinese medicine - herba epimedii, has been widely loaded to scaffolds for the treatment of bone fracture and osteoporosis. To increase drug loading capacity, and meanwhile achieve a stable and safe drug delivery, in present study, a novel icariin derivative - thiolated icariin (Ica-SH) molecule with good cytocompatibility and bioactivity was incorporated into biphasic calcium phosphate (BCP) ceramic scaffold through lca-SH intermolecular disulfide bonds (abbr., Ica-SH/BCP) to form film coating. Compared with Ica-loaded BCP scaffolds via physical adsorption (abbr., Ica/BCP), the formation of Ica-SH intermolecular disulfide bonds in Ica-SH/BCP scaffolds led to a decreased real-time drug release concentration, and a reduced drug release rate. The released drug from Ica-SH/BCP scaffolds could promote the proliferation, migration, and osteoblastic differentiation of ovariectomized rat bone mesenchymal stem cells (OVX-rBMSCs), and up-regulate the angiogenic gene expression in human umbilical vein endothelial cells (HUVECs) in vitro. The OVX-rat femoral condyle defect model also demonstrated that Ica-SH/BCP scaffolds promoted a superior bone regeneration in osteoporotic animals than BCP ones. These findings suggest that Ica-SH is a promising drug, which can be facilely incorporated into a variety of scaffolds for repairing bone defects of patients, in particular the ones with osteoporosis. (C) 2021 The Author(s). Published by Elsevier Ltd.
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