Journal
SKELETAL MUSCLE
Volume 11, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s13395-021-00260-x
Keywords
Myogenin; Knock-in mouse; tdTOMATO; Intravital imaging; Skeletal muscle
Categories
Funding
- Institut Pasteur
- Agence Nationale de la Recherche (Laboratoire d'Excellence Revive, Investissement d'Avenir) [ANR-10-LABX-73]
- Association Francaise contre les Myopathies [20510]
- Centre National de la Recherche Scientifique
- Laboratoire d'Excellence Revive
- La Ligue Contre le Cancer
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A knock-in mouse line expressing tdTOMATO fluorescent protein from the endogenous Myogenin locus was reported, allowing tracking and isolation of MYOGENIN(+) cell population. tdTOMATO fluorescence could track differentiating myoblasts both in vitro and in vivo. Live imaging showed that a fraction of the MYOGENIN(+) population could undergo one round of cell division.
Background Myogenin is a transcription factor that is expressed during terminal myoblast differentiation in embryonic development and adult muscle regeneration. Investigation of this cell state transition has been hampered by the lack of a sensitive reporter to dynamically track cells during differentiation. Results Here, we report a knock-in mouse line expressing the tdTOMATO fluorescent protein from the endogenous Myogenin locus. Expression of tdTOMATO in Myog(ntdTom) mice recapitulated endogenous Myogenin expression during embryonic muscle formation and adult regeneration and enabled the isolation of the MYOGENIN(+) cell population. We also show that tdTOMATO fluorescence allows tracking of differentiating myoblasts in vitro and by intravital imaging in vivo. Lastly, we monitored by live imaging the cell division dynamics of differentiating myoblasts in vitro and showed that a fraction of the MYOGENIN(+) population can undergo one round of cell division, albeit at a much lower frequency than MYOGENIN(-) myoblasts. Conclusions We expect that this reporter mouse will be a valuable resource for researchers investigating skeletal muscle biology in developmental and adult contexts.
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