Journal
STEM CELL REPORTS
Volume 16, Issue 2, Pages 264-280Publisher
CELL PRESS
DOI: 10.1016/j.stemcr.2020.12.019
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Funding
- NIH [U01 MH103365, R01 MH109648]
- Harris Family Professorship fund
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In this study, transcriptome and cellular phenotypes of telencephalic organoids (ORGs) and monolayers (MONs) derived from induced pluripotent stem cells (iPSCs) were compared. The results revealed increased proliferation and altered cellular characteristics in MONs, suggesting potential differences in signaling pathways and developmental processes when compared to ORGs.
Organoids (ORGs) are increasingly used as models of cerebral cortical development. Here, we compared transcriptome and cellular phe-notypes between telencephalic ORGs and monolayers (MONs) generated in parallel from three biologically distinct induced pluripotent stem cell (iPSC) lines. Multiple readouts revealed increased proliferation in MONs, which was caused by increased integrin signaling. MONs also exhibited altered radial glia (RG) polarity and suppression of Notch signaling, as well as impaired generation of intermediate progenitors, outer RG, and cortical neurons, which were all partially reversed by reaggregation of dissociated cells. Network analyses re-vealed co-clustering of cell adhesion, Notch-related transcripts and their transcriptional regulators in a module strongly downregulated in MONs. The data suggest that ORGs, with respect to MONs, initiate more efficient Notch signaling in ventricular RG owing to preserved cell adhesion, resulting in subsequent generation of intermediate progenitors and outer RG, in a sequence that recapitulates the cortical ontogenetic process.
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