Genome-wide identification and characterization of the OFP gene family in Chinese cabbage (Brassica rapa L. ssp. pekinensis)

Ovate family proteins (OFPs) are a class of proteins with a conserved OVATE domain that contains approximately 70 amino acid residues. OFP proteins are plant-specific transcription factors that participate in regulating plant growth and development and are widely distributed in many plants. Little is known about OFPs in Brassica rapa to date. We identified 29 OFP genes in Brassica rapa and found that they were unevenly distributed on 10 chromosomes. Intron gain events may have occurred during the structural evolution of BraOFP paralogues. Syntenic analysis verified Brassica genome triplication, and whole genome duplication likely contributed to the expansion of the OFP gene family. All BraOFP genes had light responsive- and phytohormone-related cis-acting elements. Expression analysis from RNA-Seq data indicated that there were obvious changes in the expression levels of six OFP genes in the Brassica rapa hybrid, which may contribute to the formation of heterosis. Finally, we found that the paralogous genes had different expression patterns among the hybrid and its parents. These results provide the theoretical basis for the further analysis of the biological functions of OFP genes across the Brassica species.

Automated summary

Ovate family proteins (OFPs) are plant-specific transcription factors involved in regulating plant growth and development. A study on Brassica rapa identified 29 OFP genes unevenly distributed on different chromosomes, with potential intron gain events. Whole genome duplication likely contributed to the expansion of the OFP gene family, with significant changes in the expression levels of these genes observed in hybrids, possibly contributing to heterosis formation. Paralogous genes displayed different expression patterns among hybrids and their parents, providing a theoretical basis for further analysis of OFP gene functions in Brassica species.