Journal
PEERJ
Volume 9, Issue -, Pages -Publisher
PEERJ INC
DOI: 10.7717/peerj.10645
Keywords
Bacteriophage; Genomics; Urinary tract infection; Proteus; Prolate
Categories
Funding
- National Science Foundation [DBI-1565146]
- Center for Phage Technology (CPT), an Initial University Multidisciplinary Research Initiative - Texas AM University
- Texas AgriLife
- Department of Biochemistry and Biophysics
- NIH [1S10RR025111-01]
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Characterization of the Proteus mirabilis bacteriophage Privateer isolated from Texas wastewater treatment plant activated sludge revealed genetic features and evolutionary relationships of structural proteins with other prolate podophages.
The Gram-negative bacterium Proteus mirabilis causes a large proportion of catheter-associated urinary tract infections, which are among the world's most common nosocomial infections. Here, we characterize P. mirabilis bacteriophage Privateer, a prolate podophage of the C3 morphotype isolated from Texas wastewater treatment plant activated sludge. Basic characterization assays demonstrated Privateer has a latent period of similar to 40 min and average burst size around 140. In the 90.7 kb Privateer genome, 43 functions were assigned for the 144 predicted protein-coding genes. Genes encoding DNA replication proteins, DNA modification proteins, four tRNAs, lysis proteins, and structural proteins were identified. Cesium-gradient purified Privateer particles analyzed via LC-MS/MS verified the presence of several predicted structural proteins, including a longer, minor capsid protein apparently produced by translational frameshift. Comparative analysis demonstrated Privateer shares 83% nucleotide similarity with Cronobacter phage vB_CsaP_009, but low nucleotide similarity with other known phages. Predicted structural proteins in Privateer appear to have evolutionary relationships with other prolate podophages, in particular the Kuraviruses
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