Journal
NANOMATERIALS
Volume 11, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/nano11020502
Keywords
surface functionalization; maghemite; cell viability; cancer disease; molecular modeling
Categories
Funding
- ANR (Agence Nationale de la Recherche)
- CGI (Commissariat a l'Investissement d'Avenir) [ANR-11-LABX-0021-01]
- Labex SEAM [ANR 11 LABX 086, ANR 11 IDEX 05 02, ANR-13-SECU-0001]
Ask authors/readers for more resources
TRAIL is a promising agent for cancer therapy as it can induce apoptosis in tumor cells while sparing normal cells. Attaching TRAIL to nanoparticles enhanced its therapeutic effect, with CO-TRAIL@NPs-NH showing a stronger pro-apoptotic effect. Computational studies indicated that attachment to nanoparticles does not affect DR4 recognition.
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the TNF cytokine superfamily. TRAIL is able to induce apoptosis through engagement of its death receptors DR4 and DR5 in a wide variety of tumor cells while sparing vital normal cells. This makes it a promising agent for cancer therapy. Here, we present two different ways of covalently grafting TRAIL onto maghemite nanoparticles (NPs): (a) by using carboxylic acid groups of the protein to graft it onto maghemite NPs previously functionalized with amino groups, and (b) by using the amino functions of the protein to graft it onto NPs functionalized with carboxylic acid groups. The two resulting nanovectors, NH-TRAIL@NPs-CO and CO-TRAIL@NPs-NH, were thoroughly characterized. Biological studies performed on human breast and lung carcinoma cells (MDA-MB-231 and H1703 cell lines) established these nanovectors are potential agents for cancer therapy. The pro-apoptotic effect is somewhat greater for CO-TRAIL@NPs-NH than NH-TRAIL@NPs-CO, as evidenced by viability studies and apoptosis analysis. A computational study indicated that regardless of whether TRAIL is attached to NPs through an acid or an amino group, DR4 recognition is not affected in either case.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available