Journal
ENVIRONMENTAL RESEARCH
Volume 151, Issue -, Pages 618-627Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.envres.2016.08.034
Keywords
Cyanobacteria; 2-methylisoborneol (2-MIB); 2-MIB-synthesis gene (mibC gene); Taste and odor; Real-time PCR
Funding
- Taiwan Ministry of Science and Technology [NSC 102-2119-M-002-008, MOST 103-2119-M-002-004, MOST 104-2119-M-002-001]
- projects of Water Resources Agency, Taiwan Ministry of Economic Affairs [MOEAWRA1020152, MOEAWRA1030051, MOEAWRA1040257]
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2-Methylisoborneol (2-MIB) is a commonly detected cyanobacterial odorant in drinking water sources in many countries. To provide safe and high-quality water, development of a monitoring method for the detection of 2-MIB-synthesis (mibC) genes is very important. In this study, new primers MIBS02F/R intended specifically for the mibC gene were developed and tested. Experimental results show that the MIBS02F/R primer set was able to capture 13 2-MIB producing cyanobacterial strains grown in the laboratory, and to effectively amplify the targeted DNA region from 17 2-MIB-producing cyanobacterial strains listed in the literature. The primers were further coupled with a TaqMan probe to detect 2-MIB producers in 29 drinking water reservoirs (DWRs). The results showed statistically significant correlations between mibC genes and 2-MIB concentrations for the data from each reservoir (R-2=0.413-0.998; p < 0.05), from all reservoirs in each of the three islands (R-2=0.302-0.796; p < 0.01), and from all data of the three islands (R-2=0.473-0.479; p < 0.01). The results demonstrate that the real-time PCR can be an alternative method to provide information to managers of reservoirs and water utilities facing 2-MIB-related incidents. (C) 2016 Elsevier Inc. All rights reserved.
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