Journal
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Volume -, Issue 168, Pages -Publisher
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/62062
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Estimation of dopaminergic neuron number in the substantia nigra is crucial for Parkinson's disease research. This study introduces an image analysis platform for accurate quantification and demonstrates its efficiency in detecting changes in neuron number. The method provides a time-efficient and accurate process for evaluating the effects of interventions on cell survival.
Estimation of the number of dopaminergic neurons in the substantia nigra is a key method in pre-clinical Parkinson's disease research. Currently, unbiased stereological counting is the standard for quantification of these cells, but it remains a laborious and time-consuming process, which may not be feasible for all projects. Here, we describe the use of an image analysis platform, which can accurately estimate the quantity of labeled cells in a pre-defined region of interest. We describe a step-by-step protocol for this method of analysis in rat brain and demonstrate it can identify a significant reduction in tyrosine hydroxylase positive neurons due to expression of mutant alpha-synuclein in the substantia nigra. We validated this methodology by comparing with results obtained by unbiased stereology. Taken together, this method provides a time-efficient and accurate process for detecting changes in dopaminergic neuron number, and thus is suitable for efficient determination of the effect of interventions on cell survival.
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