The Effects of Total Dissolved Carbon Dioxide on the Growth Rate, Biochemical Composition, and Biomass Productivity of Nonaxenic Microalgal Polyculture

The biosequestration of CO2 using microalgae has emerged as a promising means of recycling CO2 into biomass via photosynthesis, which could be used to produce biofuels as an attractive approach to CO2 mitigation. We investigated the CO2 fixation capability of the native nonaxenic microalgal culture using a 2 L photobioreactor operated in batch mode. The cultivation was carried out at varying concentrations of total dissolved CO2 (Tco(2)) in the bulk media ranging from 200 to 1000 mg L--1,L- and the temperature and light intensities were kept constant. A maximum CO2 fixation rate was observed at 400 mg L-1 of Tco(2). Characteristic growth parameters such as biomass productivity, specific growth rate, maximum biomass yield, and biochemical parameters such as carbohydrate, protein, and lipids were determined and discussed. We observed that the effect of CO2 concentration on growth and biochemical composition was quite significant. The maximum biomass productivity was 22.10 +/- 0.70 mg L-1 day(-1), and the rate of CO2 fixation was 28.85 +/- 3.00 mg L-1 day(-1) at 400 mg L-1 of Tco(2). The maximum carbohydrate (8.17 +/- 0.49% dry cell weight) and protein (30.41 +/- 0.65%) contents were observed at 400 mg L-1, whereas the lipid content (56.00 +/- 0.82% dry cell weight) was the maximum at 800 mg L-1 of Tco(2) in the bulk medium.

Automated summary

The study showed that the concentration of CO2 has a significant impact on both growth and biochemical composition. The maximum biomass productivity and CO2 fixation rate were observed at 400 mg L-1 of Tco(2), with values of 22.10 +/- 0.70 mg L-1 day(-1) and 28.85 +/- 3.00 mg L-1 day(-1) respectively. The highest carbohydrate and protein contents were observed at 400 mg L-1, while the maximum lipid content was at 800 mg L-1 of Tco(2) in the bulk medium.