Journal
JOURNAL OF OCEAN UNIVERSITY OF CHINA
Volume 20, Issue 2, Pages 383-392Publisher
OCEAN UNIV CHINA
DOI: 10.1007/s11802-021-4516-7
Keywords
interferon-induced protein with tetratricopeptide repeats 1 (IFIT1); Epinephelus lanceolatus; tetratricopeptide repeats (TPR) motif; expression pattern; antiviral function
Categories
Funding
- Shandong Breeding Project [2016LZGC009]
- Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao) [2018-MFS-T08, 2017ASTCP-OS15]
- Central Public-interest Scientific Institution Basal Research Fund, CAFS [2020TD20]
- Central Public-Interest Scientific Institution Basal Research Fund, YSFRI, CAFS [20603022018026]
Ask authors/readers for more resources
ELIFIT1 gene plays an important antiviral role in Epinephelus lanceolatus, with high expression in blood and immunity-related tissues. Upon viral infection, its expression is significantly upregulated and reaches peak levels within a specific timeframe.
Interferon-induced protein with tetratricopeptide repeats 1 (IFIT1), also known as interferon-induced protein 56 (IFI56) or Interferon-stimulated protein 56 (ISG56), was originally identified as a protein induced upon treatment with interferon and inhibited by viral replication and translational initiation. In this study, Epinephelus lanceolatus IFIT1 (ELIFIT1) gene was cloned for the first time. The complete cDNA of ElIFIT1 gene includes 2921 nucleotides, and encodes a 437-amino acid (AA) protein. The putative ELIFIT1 protein has 9 TRP domains and is highly similar with IFIT1 proteins in other teleosts. In healthy fish, ELIFIT1 gene was highly expressed in the blood, which indicate its specific function in the peripheral immune system. Its expression was also observed in various immunity-related tissues including spleen, intestine, and kidney, Inducted with spotted knifejaw iridovirus (SKIV), ELIFIT1 gene expression was upregulated in the spleen, kidney, and liver 24 h after induction and reached its peak at 72 h, indicating that ELIFIT1 may play an important role in antivirus. These findings contribute to the understanding of the antiviral regulation of ELIFIT1 gene in teleost.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available