Journal
BRAIN & DEVELOPMENT
Volume 37, Issue 8, Pages 747-752Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.braindev.2014.12.001
Keywords
Brain; Hypoxia ischemia; Neonate; Toll-like receptor 2; Toll-like receptor 4
Categories
Funding
- Research Fund for the Doctoral Program of Higher Education of China [20100071120098]
- Shanghai Rising-Star Program [11QA1400700]
Ask authors/readers for more resources
Aim of the study: Recent reports provide evidence that Toll-like receptors (TLRs) play a crucial role in cerebral ischemic injuries and neuronal cell death. The precise role of TLRs in mediating neuronal damage remains to be fully elucidated. In this study, we investigated the expression of TLR2 and TLR4 in the hippocampus of the neonatal rats. Materials and methods: Postnatal day 7 Sprague Dawley rats were used. The hypoxia ischemia brain injury models were made by ligature of the left common carotid artery and then inhalation of 8%O-2/92%N-2 for 2 h. TLR2 and TLR4 expression was assessed with immunohistochemical staining and Western blot assays at 3 d, 7 d, and 14 d after injury. Results: At the 3 d and 7 d time points, we found a significant increase in the number of TLR2 and TLR4 positive cells in the hippocampus of the HI group when compared with the sham group (P < 0.01). Western blot showed similar results. Conclusions: The study indicates that TLR2 and TLR4 are involved in the hypoxic ischemic injury of full-term neonatal brains. (C) 2014 The Japanese Society of Child Neurology. Published by Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available